Micro-chips and micro-fluidics

Exploded ViewThe miniaturization of the electrodes using techniques borrowed from the semiconductor industry and their integration in a continuous flow micro-cell opens wide and interesting perspectives for the development of biosensors, offering the possibility to increase both the speed and the sensitivity of detection. Such a micro-cell, that works in continuous flow, has been previously developed by the authors. Its functional tests have revealed some characteristics that should be improved: (a) sensitivity, (b) the reliability of both electric and fluidic connections. The causes of these drawbacks have been analyzed and new technical solutions have been adopted having as a result a new engineered device. The electrochemical biosensor micro-cell consists of a set of working electrodes (WE), a counter-electrode (CE), and a reference electrode (RE) placed inside a reservoir provided with inlet and outlet openings. Electric connections allow the incoming and outgoing electric signals to be transmitted between the micro-electrodes and the external control unit. Fluidic connections allow the analyte solution to flow through the reservoir placed on the suction side of an external peristaltic pump.

Engineering a continuous flow electrochemical micro-cell for biosensor application: new achievements M Ilie, R Dejana, V Foglietti, R Renda, L Nardi, A Masci, B Lanza, MR Montereali, L.Della Seta, W.Vastarella, R.Pilloton - Intern. J. Environ. Anal. Chem. Vol. 90, No. 1, 15 January 2010, 31–39

Developing a miniaturized continuous flow electrochemical cell for biosensor applications - M.Ilie, E.Ovreiu, R.Dejana, V.Foglietti, L.Nard, A.Masc ı,B.Lanza, L.Della Seta, MR.Montereali, W.Vastarella, R.Pılloton - Adv. Opto-, Micro-electronics, and Nanotechnologies, SPIE Vol. 72974 2009

Continuous flow micro-cell for electrochemical addressing of engineered bio-molecules - J Maly, M Ilie, V Foglietti, E Cianci, A Minotti, L Nardi, A Masci, W.Vastarella and R.Pilloton - Sensors and Actuators B: Chemical (2005) 111, 317-322

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